DNA_paint

Theory Task

Memo

• DNA structure has merit. We can design desired shape molecules by using base pair of DNA. By hacking DNA base pair structure and sequence, it is possible to design variety of shapes and this technology is called DNA origami.
• DNA origami technology is being constructed by single strand DNA and Complementary DNA.
• By controlling these two elements, we can control bending structure of DNA. This concept is DNA origami and possible to make the design of 3D nano structure.

DNA Origami - 1	DNA Origami - 2

DNA Origami

• And caDNAno is usable interface to design DNA Origami. According to "Rapid prototyping of 3D DNA-origami shapes with caDNAno / doi:10.1093"

caDNAno

‘scaffold’ strand can be employed to template the assembly of hundreds of oligonucleotide ‘staple’ strands

‘scaffolded DNA origami’ method to producing 3D shapes

caDNAno, an open-source soft- ware package with a graphical user interface that aids in the design of DNA sequences for folding 3D honeycomb-pleated shapes

software is available at http://cadnano.org/

• DNA Jigsaw is related technology with DNA origami. This connects several 2D DNA parts and making frame structures.

DNA Jigsaw

Lab Task

• Due to wet-ware limitation, we will hold experiment later.